全文获取类型
收费全文 | 10936篇 |
免费 | 1103篇 |
国内免费 | 1583篇 |
出版年
2023年 | 169篇 |
2022年 | 141篇 |
2021年 | 268篇 |
2020年 | 427篇 |
2019年 | 436篇 |
2018年 | 356篇 |
2017年 | 422篇 |
2016年 | 407篇 |
2015年 | 475篇 |
2014年 | 565篇 |
2013年 | 789篇 |
2012年 | 537篇 |
2011年 | 472篇 |
2010年 | 461篇 |
2009年 | 546篇 |
2008年 | 551篇 |
2007年 | 597篇 |
2006年 | 533篇 |
2005年 | 471篇 |
2004年 | 410篇 |
2003年 | 362篇 |
2002年 | 317篇 |
2001年 | 332篇 |
2000年 | 317篇 |
1999年 | 241篇 |
1998年 | 208篇 |
1997年 | 228篇 |
1996年 | 201篇 |
1995年 | 198篇 |
1994年 | 191篇 |
1993年 | 206篇 |
1992年 | 157篇 |
1991年 | 140篇 |
1990年 | 115篇 |
1989年 | 136篇 |
1988年 | 112篇 |
1987年 | 104篇 |
1986年 | 102篇 |
1985年 | 137篇 |
1984年 | 123篇 |
1983年 | 103篇 |
1982年 | 102篇 |
1981年 | 89篇 |
1980年 | 87篇 |
1979年 | 72篇 |
1978年 | 54篇 |
1977年 | 40篇 |
1976年 | 34篇 |
1975年 | 17篇 |
1973年 | 17篇 |
排序方式: 共有10000条查询结果,搜索用时 218 毫秒
81.
Using molecular dynamics simulations and steady‐state fluorescence spectroscopy, we have identified a conformational change in the active site of a thermophilic flavoenzyme, NADH oxidase from Thermus thermophilus HB8 (NOX). The enzyme's far‐UV circular dichroism spectrum, intrinsic tryptophan fluorescence, and apparent molecular weight measured by dynamic light scattering varied little between 25 and 75°C. However, the fluorescence of the tightly bound FAD cofactor increased approximately fourfold over this temperature range. This effect appears not to be due to aggregation, unfolding, cofactor dissociation, or changes in quaternary structure. We therefore attribute the change in flavin fluorescence to a temperature‐dependent conformational change involving the NOX active site. Molecular dynamics simulations and the effects of mutating aromatic residues near the flavin suggest that the change in fluorescence results from a decrease in quenching by electron transfer from tyrosine 137 to the flavin. Proteins 2012. © 2011 Wiley Periodicals, Inc. 相似文献
82.
83.
Effects of ambient temperature on avian incubation behavior 总被引:6,自引:1,他引:5
Ambient temperature is commonly thought to influence avian incubation
behavior. However, results of empirical studies examining correlationsbetween ambient temperature and bout duration are equivocal.We propose that these equivocal results can be partly explainedby developing a conceptual understanding of how we should expecttemperature to influence incubation. We demonstrate why linearcorrelation analyses across a wide range of temperatures canbe inappropriate based on development of an incubation model
for small birds that incorporates how ambient temperature influencesboth embryonic development and adult metabolism. We found supportfor predictions of the model using incubation data from orange-crownedwarblers (Vermivora celata) in Arizona. Both off- and on-boutduration were positively correlated with ambient temperaturebetween 9° and 26°C, but unrelated to ambient temperature<9° and 26-40°C. Bout durations declined as ambienttemperature approached or exceeded 40°C. Incubating orange-crowned
warblers appeared to avoid bouts off the nest <7 min andbouts on the nest <20 min. Time of day, duration of theprevious bout, and variation among nests all explained variationin both on- and off-bout duration. Although we found supportfor the general shape of the incubation model, temperature still
explained only a small portion of the overall variation in on-and off-bout duration. Results of previous studies were generallyconsistent with the model for off-bout duration; most studiesin colder environments reported positive correlations withtemperature, and the one negative correlation reported was
from a hot environment. However, the relationships between on-boutduration and temperature reported in previous studies wereless consistent with our model and our data. Although somediscrepancies could be explained by considering our model,some studies reported negative correlations in cold environments.The effect of ambient temperature on duration of on-bouts probablydiffers among species based on the amount of fat reserves females
typically carry during incubation and the extent of male incubationfeeding. Additional studies of the effects of temperature onavian incubation will help improve the general model and ultimatelyaid our understanding of energetic and ecological constraintson avian incubation. 相似文献
84.
Air temperature and relative humidity have long been suspected of affecting the performance of marathon runners. Though these factors are important in their extremes, we show that other factors are even more indicative of race performances. Performances of the top 3 finishers in the last 30 Boston Marathons were correlated with hourly meteorological data for each race day. These 90 individual performances were classified as: record breaking performances (31), average performances (35), and unusually slow performances (24). The factors that help predict record breaking and unusually slow performances are: (i) wet bulb temperature, (ii) percent sky cover, and (iii) presence or absence of a light precipitation. Record breaking performances are characterized by a wet bulb temperature of <7.8°C, and 100% sky cover. A light drizzle is also conducive to better performances. On the other hand, unusually low performances are accompanied by a wet bulb temperature of >7.8°C, and a sky cover of 50% or less. No light, precipitation was recorded on any of the unusually slow race days. A graphic analysis clearly shows these relationships to exist. In addition, a multiple regression analysis confirms the importance of these variables. The authors advise that these are reliable predictors; however, when considering marathon races held in various geographical regions and differing climatic regimes, the exact numerical thresholds used here may not apply. 相似文献
85.
The effect of incubation and rearing temperature on muscle development and swimming endurance under a high-intensity swimming test was investigated in juvenile Chinook salmon (Oncorhynchus tshawytscha) in a hatchery experiment. After controlling for the effects of fork length (LF) and parental identity, times to fatigue of fish were higher when fish were incubated or reared at warmer temperatures. Significant differences among combinations of pre- and post-emergence temperatures conformed to 15–15°C > 15–9°C > 9–9°C > 7–9°C > 7–7°C in 2011 when swimming tests were conducted at 300 accumulated temperature units post-emergence and 15–9°C > (7–9°C = 7–7°C) in 2012 when swimming tests were conducted at an LF of c. 40 mm. The combination of pre- and post-emergence temperatures also affected the number and size of muscle fibres, with differences among temperature treatments in mean fibre cross-sectional area persisting after controlling for LF and parental effects. Nonetheless, neither fibre number nor fibre size accounted for significant variation in swimming endurance. Thus, thermal carryover effects on swimming endurance were not mediated by thermal imprinting of muscle structure. This is the first study to test how temperature, body size and muscle structure interact to affect swimming endurance during early development in salmon. 相似文献
86.
87.
Gunnar ?hlund Per Hedstr?m Sven Norman Catherine L. Hein G?ran Englund 《Proceedings. Biological sciences / The Royal Society》2015,282(1799)
The temperature dependence of predation rates is a key issue for understanding and predicting the responses of ecosystems to climate change. Using a simple mechanistic model, we demonstrate that differences in the relative performances of predator and prey can cause strong threshold effects in the temperature dependence of attack rates. Empirical data on the attack rate of northern pike (Esox lucius) feeding on brown trout (Salmo trutta) confirm this result. Attack rates fell sharply below a threshold temperature of +11°C, which corresponded to a shift in relative performance of pike and brown trout with respect to maximum attack and escape swimming speeds. The average attack speed of pike was an order of magnitude lower than the escape speed of brown trout at 5°C, but approximately equal at temperatures above 11°C. Thresholds in the temperature dependence of ecological rates can create tipping points in the responses of ecosystems to increasing temperatures. Thus, identifying thresholds is crucial when predicting future effects of climate warming. 相似文献
88.
1. Recent observations of actuarial senescence – an increase in mortality rate with age – have challenged the assertion that the brevity of adult insect life spans precludes ageing. 2. Here the rate of senescence in 22 species of Lepidoptera was quantified by fitting demographic models to adult survivorship data drawn from a range of field and laboratory studies. 3. Senescence was evident in all 22 species investigated, with a model of age‐related mortality consistently fitting the survivorship curves significantly better than an alternative model which assumes constant mortality. 4. The rates of senescence varied significantly among species. The rates of senescence also differed significantly between sexes for all species tested, but not in a consistent way. 相似文献
89.
90.
The life-cycle of the ancient asexual ostracod Darwinula stevensoni was studied during 1 year in a eutrophic pond in Belgium. The reproductive period of this species started in March and was effectively completed by September of the same year. All changes in population structure took place during the spring and summer months and a rapid turnover of the instars was observed. The life-cycle of Darwinula stevensoni appears to take one year or less in Belgium and this is considerably shorter than the 4 years which had been reported previously from subarctic populations. The difference to the present study is most likely temperature-related. Maximal densities of D. stevensoni were observed in June and July and attained 105 ind. m–2. During winter, densities were lower with a mean of 104 ind. m–2. Consequently, the calculated population size of each month was high throughout the year. Together with the low mutation rate, such a large population size could effectively counteract the stochastic loss of mutation-free genotypes as predicted by Muller's ratchet. D. stevensoni is a brooder; the maximum number of embryos and juvenile instars (up to third stage) found within a single female was 11. 相似文献